Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Absorbance Filter, 515 nm, 8 nm
Absorbance bandpass filter suitable for microplate absorbance assays. Centered at 515 nm with an 8 nm bandwidth, this optical filter installs in filter slide slots and provides narrowband absorbance detection for plate-based assays. Compatible with FilterMax F3 and F5 multimode microplate readers.
- Center wavelength 515 nm.
- Bandwidth 8 nm.
- Filter type Absorbance bandpass.
- Application Microplate absorbance measurements.
- Form factor Slide-mounted filter for filter-based readers.
Electron Microscopy Sciences NIGHTSEA™ Stereo Fluorescence Add-On Light and Filter Set, Ultra Violet
Additional combinations excitation/ emission wavelength combination can be added NIGHTSEA™ Stereo Microscope Fluorescence Viewing system by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. Sets are named and color coded for the color of the excitation light, not the color of the emitted fluorescence.
Color: Ultra Violet
Excitation: 360-380nm
Emission filter: 415 nm longpass
Fluorophores: DAPI
Sigma Aldrich Fine Chemicals Biosciences Hellma(R) fluorescence cuvettes, semi Micro Suprasil(R) quartz, spectral range 200-2500 nm, pathlength 10x4 mm, chamber volume 1000 muL | 1EA
Hellma(R) fluorescence cuvettes, semi Micro Suprasil(R) quartz, spectral range 200-2500 nm, pathlength 10x4 mm, chamber volume 1000 muL | 1EA
Molecular Devices Multi-Mode Microplate Reader, Dual Monochromator, 6-384-Well
The SpectraMax M3 multi-mode microplate reader is a configurable, high-performance instrument for absorbance, fluorescence, and luminescence assays, designed for both research and regulated laboratory environments. It combines tunable optics with flexible plate handling and compliance-capable analysis software.
- Dual monochromator optics, tunable in 1-nm increments
- Detection modes include absorbance, fluorescence, luminescence, fluorescence polarization, and time-resolved assays
- Supports 6- to 384-well microplates, with top and bottom reading options
- High-sensitivity detectors with xenon flash lamp illumination
- Software features secure data acquisition and 21 CFR Part 11-compatible controls
Enterprise Technology Solutions Ergotron LX Desk Mount Monitor Arm (Matte Black)
Ergotron LX Desk Mount Monitor Arm (Matte Black)
Molecular Devices Microplate Reader, Multi-Mode, Tunable Monochromator, 6-384 Well
The SpectraMax iD3 multi-mode microplate reader from Molecular Devices provides absorbance, fluorescence (top and bottom), and luminescence detection for 6-384 well microplates. It features a tunable monochromator with 1-nm increments, intuitive touchscreen control, and software tools for spectral scanning and assay optimization, enabling rapid assay development and reliable quantitative measurements.
- Multi-mode detection for absorbance, fluorescence, and luminescence assays.
- Tunable monochromator with 1-nm wavelength increments for spectral flexibility.
- Broad wavelength coverage for diverse assay types, including UV and visible ranges.
- Supports 6 to 384 well plate formats for assay throughput flexibility.
- Intuitive touchscreen and software tools for quick setup and assay optimization.
- High photometric precision and repeatability for reliable quantitative results.
Revvity Health Sciences Inc HTRF Human Total IKZF3 Detection Kit, 10,000 Assay Points
HTRF Human Total IKZF3 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK3 Detection Kit, 10,000 Assay Points
HTRF Human Total JAK3 Detection Kit, 10,000 Assay Points
Molecular Devices Fluorescence Filter, 425 nm, 40 nm, With Holder
Molecular Devices fluorescence filter with holder provides a 425 nm central wavelength and 40 nm bandwidth for selective optical isolation in fluorescence, luminescence, and time-resolved fluorescence (TRF) assays. The filter comes mounted in a holder for integration into compatible multi-mode microplate readers and imaging modules.
- Designed for fluorescence, luminescence, and TRF assays.
- 425 nm central wavelength with 40 nm bandwidth.
- Supplied in a holder for instrument mounting.
- Reduces background signal and improves specificity.
- Suitable for integration with multi-mode microplate readers and imaging systems.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total ErbB3 (HER3) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Total ErbB3 (HER3) Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human Total SALL4 Detection Kit, 50,000 Assay Points
HTRF Human Total SALL4 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total FGFR1 Detection Kit, 10,000 Assay Points
HTRF Human Total FGFR1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF SARS-Cov2 Spike S1 protein detection kit, 500 Assay Points
HTRF SARS-Cov2 Spike S1 protein detection kit, 500 Assay Points
Molecular Devices Fluorescence Filter, 465 nm, 35 nm, With Holder
Molecular Devices fluorescence band-pass filter supplied with a holder, designed to provide selective optical filtering for fluorescence assays. The filter has a center wavelength of 465 nm and a nominal full width of 35 nm, enabling targeted detection of blue/green fluorophores in multi-mode microplate reader applications.
- Provides a center wavelength of 465 nm for blue/green fluorophore detection.
- 35 nm full width for controlled spectral selection and reduced cross-talk.
- Includes a holder for secure mounting in the instrument filter tray or slide slot.
- Intended for fluorescence detection in multi-mode microplate assays.
- Helps reduce background and improve signal specificity in fluorescence measurements.