Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Micro-Volume Microplate Kit, HTS, 24-Well and 64-Well, 2 μL/4 μL
The SpectraDrop High-Throughput Screening (HTS) Kit provides consumables and a microplate adapter to enable automated, low-volume sample measurement using micro-volume microplates. It combines multiple low-volume slide formats and cover slides to support high-throughput nucleic acid and absorbance assays with minimal sample consumption.
- Contains one microplate adapter for automated plate handling.
- Includes five 24-well low-volume bottom slides.
- Includes five 64-well low-volume bottom slides.
- Includes five 2 μL cover slides and five 4 μL cover slides.
- Supports 2 μL and 4 μL sample volumes (approx. 0.5 mm and 1 mm pathlengths).
- Suitable for high-throughput nucleic acid quantitation and absorbance measurements.
Molecular Devices Optical Filter, Photometric, 580 nm, Narrow Bandwidth
Molecular Devices AD 340 photometric filter (6590-0022) is a replacement optical filter intended for use in compatible Molecular Devices microplate readers. It provides wavelength selection for photometric and absorbance measurements and is installed in the instrument filter holder or slide.
- Replacement optical element for compatible microplate readers.
- Provides narrow wavelength selection for photometric and absorbance assays.
- Installs into standard filter holder or slide for easy replacement.
- Designed to reduce background signal and improve assay specificity.
- Intended for laboratory use with microplate-based assays.
Molecular Devices Photometric Filter, 650 nm, Absorbance
The AD 340 Photometric Filter 650 is a replacement photometric filter for FilterMax F3 and F5 multimode microplate readers from Molecular Devices. It provides a defined optical band centered at 650 nm for absorbance measurements and is supplied as a single unit for instrument maintenance and assay flexibility.
- Provides a defined optical band centered at 650 nm for absorbance measurements.
- Designed for reliable photometric performance in routine assays.
- Supplied as a single replacement unit to simplify inventory and maintenance.
- Easy to install into compatible instrument filter holders for quick swaps.
Dojindo Molecular Technologies Inc ATP Assay Kit-Luminescence (50 tests) is used to determine intracellular levels of ATP by luciferase luminescence assay. High-sensitivity detection can be performed with stable luminescence. 50 tests
Adenosine triphosphate (ATP) is an energy source for living cells and is synthesized by both glycolysis and mitochondrial oxidative phosphorylation. Decreased ATP levels are associated with cancer, aging, neurodegenerative diseases, and mitochondrial diseases. The ATP Assay Kit-Luminescence enables the quantitation of intracellular ATP by luciferase luminescence assay. This kit can be applied for microplate assay. Without a complicated process including medium removal and cell washing, the ATP Assay Kit-Luminescence can generate a stable luminescent signal with a half-life of greater than 3 hours by simply mixing the kit components and adding the reagent. This kit contains an ATP Standard, which provides highly reproducible data without measuring unstable ATP. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Molecular Devices Fluorescence Filter, 535 nm, 25 nm, With Holder
Molecular Devices fluorescence emission filter with integrated holder provides a narrow 535 nm center wavelength and 25 nm bandwidth for selective detection of fluorophores emitting near 535 nm. It is intended for filter-based microplate readers and supports fluorescence and TR-FRET assay detection, including TAMRA-based assays.
- Center wavelength 535 nm.
- Bandwidth 25 nm.
- Includes integrated holder for installation in filter slot.
- Suitable for fluorescence and TR-FRET detection workflows.
- Designed for use in filter-slot multi-mode microplate readers.
Molecular Devices Fluorescence Polarization Filter, 360 nm, 35 nm, Polarized
This fluorescence polarization filter is designed for fluorescence polarization assays in microplate readers and includes a holder for secure installation into filter slide slots. It provides a 360 nm excitation center with a 35 nm bandwidth and a vertically polarized element to support FP measurements.
- 360 nm excitation center with 35 nm bandwidth
- Vertical polarization for fluorescence polarization measurements
- Includes holder for secure installation in filter slide slot
- Enables selective excitation and reduced background signal for FP assays
Molecular Devices Fluorescence Polarization Filter, 360 nm, 35 nm, Horizontal
Fluorescence polarization filter slide with integrated holder for microplate readers. Optimized for excitation at 360 nm with a 35 nm optical bandwidth and horizontal polarization orientation, this filter is used in fluorescence polarization assays to measure molecular binding and rotational mobility.
- Designed for fluorescence polarization measurements
- Optimized excitation wavelength 360 nm with 35 nm bandwidth
- Horizontal polarization orientation for emission analysis
- Includes an integrated holder for secure installation in filter slides
- Compatible with multi-mode microplate readers that accept filter slides
Molecular Devices Fluorescence Emission Filter, 550 nm, 10 nm
Emission filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), intended for selective fluorescence detection in multi-mode microplate readers that accept standard filter slides or mounts.
- Center wavelength 550 nm.
- Bandwidth 10 nm (FWHM).
- Emission bandpass design for fluorescence detection.
- Reduces spectral crosstalk and improves signal specificity.
- Suitable for use in multi-mode microplate readers and compatible filter slide systems.
Molecular Devices Excitation Filter, 485 nm, 20 nm Bandwidth
Excitation filter for fluorescence microplate assays centered at 485 nm with a 20 nm bandwidth. The filter is designed for installation in filter slide holders and is used to selectively excite blue-range fluorophores in fluorescence intensity and ELISA workflows.
- Precise 485 nm center wavelength for blue-excited fluorophores.
- 20 nm bandwidth for selective excitation and reduced background signal.
- Designed for installation in filter slide holders used by multi-mode microplate readers.
- Suitable for fluorescence intensity assays and ELISA workflows.
- Replaceable optical element for routine maintenance and assay optimization.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Vertical
The Molecular Devices fluorescence polarization filter (MPN 6590-0094) is a vertical polarization filter engineered for fluorescence polarization assays with a 485 nm center wavelength and a 20 nm optical bandwidth. It is intended as a replacement or accessory filter for compatible multi-mode microplate readers.
- Optimized for fluorescence polarization assays at a 485 nm center wavelength.
- 20 nm bandwidth provides narrow spectral selection and reduced crosstalk.
- Vertical polarization orientation for polarization-based measurements.
- Designed for installation in filter-slide assemblies of compatible microplate readers.
- Replacement filter for restoring or modifying detection channels.
Molecular Devices Luminescence Filter, 515 nm, 30 nm, Holder
Luminescence emission filter and holder designed to isolate acceptor emission near 515 nm for sensitive luminescence and BRET measurements on compatible microplate readers.
- Luminescence emission filter with holder.
- Center wavelength 515 nm.
- Bandwidth 30 nm.
- Application acceptor emission detection, including BRET assays.
- Intended for use in compatible microplate reader filter holders.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Cyclin A2 Detection Kit, 100 Assay Points
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